How to extract plant protein?

How to extract protein from plant tissue
Methods for protein extraction from plant tissues trichloroacetic acid acetone precipitation method grind leaves in liquid nitrogen, add 3 times the volume of the sample, overnight at -20 ℃, and then centrifuge (4 ℃ at 8000rpm for more than 1 hour) to discard the supernatant. Add equal volume of ice bath acetone (containing 0.07% of β- Mercaptoethanol)How to pretreat protein extracted from wheat leaves
Different extraction methods have different treatment methods, degrees and requirements for samples, and the results are also different. （1） Extract leaf protein by (NH4) 2SO4 precipitation method take 0.3g plant leaf protein, grind it with liquid nitrogen, transfer it into a centrifuge tube, add 3ml extraction buffer, shake it well and extract it at 4 ℃ for 1HHow to extract protein from plant tissue
Plant tissue protein extraction method: according to the sample weight (add 3.5ml extraction solution to 1g sample, which can be appropriately added according to different materials), prepare the extraction solution and put it on ice Put the sample in a mortar and grind it with liquid nitrogen. After grinding, add it to the extraction solution and let it stand on ice (3-4 hours)What are the methods of plant protein extraction
Plant tissue protein extraction method: according to the sample weight (add 3.5ml extraction solution to 1g sample, which can be appropriately added according to different materials), prepare the extraction solution and put it on ice. Put the sample in a mortar and grind it with liquid nitrogen. After grinding, add it to the extraction solution and let it stand on ice (3-4 hours)Extraction of plant leaf protein
Plant tissue protein extraction method: according to the sample weight (add 3.5ml extraction solution to 1g sample, which can be appropriately added according to different materials), prepare the extraction solution and put it on ice. Put the sample in a mortar and grind it with liquid nitrogen. After grinding, add it to the extraction solution and let it stand on ice (3-4 hours)Extraction methods of plant proteins
There are basically several methods for extracting plant proteins: salting out method, organic solvent method and isoelectric point method. Principle of salting out method: salting out method refers to the method of adding a large number of inorganic salts to the drug solution to reduce the solubility of some macromolecular substances, precipitate and separate them from other componentsHow to better extract plant protein
Leakage detection of dialysis bag: one end of the dialysis bag shall be clamped with a clamp (or tied with a rope). After filling with water, the dialysis bag shall be properly pressurized with a finger, and the sample can be loaded only after no leakage is found. Sample bagging: dissolve the protein with buffer solution first, and then put it into the dialysis bag. The dialysis bag should not be full, and usually one-third to one-half of the space should be reservedPlant protein extraction
Extraction method of plant protein: phenol extraction; TCA acetone precipitation briefly describe the TCA acetone precipitation I use: 1 The liquid nitrogen grinding sample is powder, 1:10 (m/v) is added with acetone containing 10%tca, and precipitated at -20 ℃ for half an hour 35000g was centrifuged for 10min, and the supernatant was removed. Add the original volume of acetone to the precipitationBasic extraction methods of plant protein from Chinese herbal medicine
Before loading, add the lysate, place it at room temperature for 30 minutes to fully dissolve the protein in the lysate, and then centrifuge (at 15 ℃ 8000rpm for 1 hour or more, the standard is no precipitation), which can be temporarily stored at 4 ℃ for use. The protein was quantified by Brandford method, and then it could be put into -80 ℃ for standbyHow to extract plant cell membrane proteins
I think the simple methods are: 1. Ultrasonic crushing, salt ion precipitation, washing and desalting; 2. Look at the size of the protein you want to extract, and dialysis with dialysis membrane or dialysis bag; 3. Run SDS PAGE, dig glue and elute